RESIPHER: turns a multi-well plate into a dynamic oxygen consumption reader.
Through the partnership with Lucid Scientific, Baker provides a suite of the most sensitive and accurate optical oxygen sensors available.
Based on a unique, patented technology, RESIPHER multiwell plate analyzers offer unmatched performance in a compact package with low-power, plug-and-play USB connectivity.
RESIPHER’s web-based, real-time logging and analysis software provides fast and easy data visualization and is extendable for customized real-time analysis.
USAGE
RESIPHER is intended to be used in an incubator or Physoxia Workstation conditions.
The sterile, disposable lids attach to the underside of the device to monitor 4 or 32 wells simultaneously.
The disposable lids are compatible with most standard 96-well plates. Lids arrive sterilized and calibrated – simply place them on the top of your plates and begin your experiment.
BENEFITS:
APPLICATIONS:
Cancer biology, angiogenesis, stroke, and brain injury, vital organ and muscle tissue monitoring, ophthalmology, wound healing as well as dissolved oxygen monitoring in cell culture, bioreactors, etc.
PRINCIPLE OF OPERATION:
RESIPHER devices utilize proprietary high-resolution optical oxygen sensors to characterize oxygen consumption and the oxygen environment in cell culture. The RESIPHER is a handheld device that rests on top of a plate in the incubator or workstation. The device interfaces a sterile/disposable lid with probes that extend into the media directly above the cells.
Probes are small enough (500μm diameter) that the system is non-invasive to the cell culture.
An oxygen concentration gradient forms in the media as a direct result of cellular oxygen consumption. The readout is attained by continually scanning the probes vertically above the cells to measure the gradient, then using sophisticated signal processing algorithms to convert concentration readings to cellular oxygen consumption.
The device streams in real-time to a web-based analysis and visualization software by way of a hub that resides outside of the incubator.
The primary readout is oxygen consumption, but the user also has access to a characterization of oxygen concentration, incubator temperature, relative humidity, and several other environmental factors.
